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I'll achieve Bachelor of Science in Biochemistry on 06/17/2017. This will be the time I finish my four college years at University of California, Santa Barbara. I'll miss here.

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About Majoring in Biochemistry

Welcome to Jiayi's Biochemical page. I am an undegraduate majoring in biochemistry (B.S) at University of California, Santa Barbara.

Four years of high quality education at UCSB empowers me the ability and knowledge in biochemical field. During four years, I have been worked at inorganic chemistry lab, organic chemistry lab and biochemical lab. Thus, I am familiar with lab techniques, concepts and related knowledge.
Biochemistry is always my passion because working a pharmaceutical company is always my dream.

I am obsessed with DNA, RNA and protein world. I have been worked at biochemical lab from September 2016 to present. I have studied the thermal denaturation of double-stranded DNA, DNA electrophoresis, protein chromatography, mutagenesis, immunodetection and other analysis. Please feel free to read my work if you are interested in.

Biochemical Skills

Raw Data Collection

Four years of working experience at wet lab empowers me ability to collect raw data efficiently and accurately. No matter what kind of data expected, I could always get them without wasting chemicals and consuming too much time.

Raw Data Analysis

I'm good at handling with different sets of data, no matter they are perfect or imperfect. I have been analyzed enzyme kinetics data, protein/DNA chromatography data using both individual calculation and computer-based calculation.

Data Presentation/Management

After finishing wet lab, showing results in an organized and easy-understanding way is my advantage. This empowers me ability to communicate with scientific world.

Data Trouble-Shooting

NO experiment design is perfect and my advantage is too trouble shoot drawbacks in experiment design in order to achieve best performance. Not only for experiment design, I'm also good at trouble-shooting any kinds of problems encountered during lab.

Electrophoresis

Gel electrophoresis is a laboratory method used to separate DNA, RNA and protein mixtures based on molecular size. It is widely applied in medical and biochemical field for DNA or protein identification. The first slide shows DNA plasmid (2629 bp) digested by two different enzymes: EcoRI and Topoisomerase 1.

Microscopy

Microscopy, an optical instrument, is used to enlarge small objects. It is the most common technique used in library to enlarge small objects. this is a collection of images observed by fluorescence microscope with different magnitude: 10x (a), 20x (b), 40x (c) and 100x (d). These images show sub-cellular components in different color. The nucleus, dyed with DAPI, is shown in blue color; actin, dyed with Texas Red-X, is in red color; the microtubules, dyed with BODIRY, is in green color.

Chromatography

Chromatography could be separate proteins based on their properties, such as size, charge, hydrophobicity, affinity-tag. This sample provides the operation method of size-exclusion chromatography, including of the packing of Toyopearl resin in a 1.0 x 30 cm column, the separation process of five different proteins, blue dextran (Mw=2,000,000), Ovalbumin (Mw=45,000), Myoglobin (Mw=16,951) and Vitamin B12.